河北省薄罗藓科植物分类的初步研究

报道了河北省薄罗藓科植物6属共12种（含1变种），其中中国新记录种1种：北方假细罗藓Pseudoleskeella papillosa(Lindb.)Kindb.；河北省新记录属2属：薄罗藓属Leskea Hedw.和褶藓属Okamuraea Broth.；河北新记录种7种：薄罗藓Leskea polycarpi Hedw.，细枝藓Lindbergia brachyptera(Mitt.)Kindb.，细枝藓疣齿变种L. brachyptera var.austinii (Sull.)Grout.,阔叶细枝藓L. brevifolia(Gao)Gao，瓦叶假细罗藓Pseudoleskeella tectorum(Brid.)Kindb.,褶藓O.hakoniensis(Mitt.)Broth.和短枝褶藓O.brachydictyon(Card.）Nog..本文根据标本编制了河北省薄罗藓科植物分属、种检索表，简要讨论了其生境行理分布特点。旨在为河北省乃至中国苔藓植物的系统研究及苔藓植物资源的开发利用提供可借鉴的资料。研究标本保存在河北师范大学植物标本室（HBNU)。     

棉铃虫抗药性监测及其化学防治

1997～1998年,用浸叶接虫法测定了山西省南部棉区永济县棉铃虫对常用5种农药的抗药性,同时进行了多种农药防治棉铃虫田间药效试验研究。结果表明,永济县棉铃虫对氯氰菊酯已产生了高等水平的抗药性,对久效磷产生了中等水平的抗药性,但对灭多威仍未产生明显的抗药性。水胺硫磷、甲基对硫磷、氯氟氰菊酯、氯氰菊酯、丙·辛乳油和甲对·氯乳油等农药防治永济县二代棉铃虫效果较好     

二氯菊酸光学纯度的毛细管气相色谱法测定研究

使用以 2 ,6 -二 -O-戊基 - 3-O-丁酰基 -β-环糊精作手性固定相制成的毛细管手性柱 ,用气相色谱法在 120℃成功地分离了外消旋二氯菊酸甲酯对映体 ,其中对顺、反外消旋二氯菊酸甲酯对映体的手性分离因子分别达到 1.03和 1.04 ,均大于测定光学纯度 (e.e.% )值所要求的 1.02。测定了 4种手性二氯菊酸甲酯的光学纯度 ,方法简便易行 ,可用于二氯菊酸的光学纯度测定     

小地老虎为害棉苗缺株产量损失及棉株群体补偿力模拟试验

研究结果明确了模拟棉苗受小地老虎为害造成断苗缺株后，周围棉株有不同程度的补偿作用，主要表现在有效铃的增加和产量的提高，缺１株苗成铃能获得１０１．８５％的补偿，籽棉平均补偿率达８７．２４％，损失率为１２．７６％。但自连续缺２株以上，则补偿率下降为５０．６２％－３５．７５％，损失率上升为４９．３７％－６４．２４％。补偿量和损失量（率）是随缺株数的增加而增长，而补偿率则随缺株数增加而下降，在不同缺株数间     

大白菜感官品质与营养品质相关性研究

本文以12个大白菜品种为试材,研究了大白菜感官品质与可溶性糖、可溶性蛋白质、维生素C、粗纤维、可溶性固形物等营养品质指标的相关性。通过逐步回归选择和通径分析,结果表明,对大白菜感官品质起主要作用的因素是可溶性糖,其次是粗纤维,再次是可溶性蛋白质。可溶性糖和可溶性蛋白质对感官品质起正向作用,而粗纤维对感官品质起负向作用。经简化的多元线性回归方程为Y=7.2408 0.4344x_1(可溶性糖％干重)-0.2979x_2(粗纤维％干重)。可以用此方程对大白菜品质做近似评价。     

青藏高原几种嵩草的生物量及其幼苗生长发育的初步研究

研究了青藏高原４种嵩草属植物的枝条发育状况，生物量积累特征以及３种嵩草幼苗的生长发良规律。结果表明；各嵩草营养枝条的季节变化有两个高峰期，而线叶蒿草的生殖枝有一个明显的高峰期，其它嵩草无高期峰。     

Whole Genome Methylation Variation Analysis of Longissimus Dorsi Between Tan Sheep and Hu Sheep

It aimed to identify the differential methylation region (DMR) and differential methylation gene (DMG) through the analysis of the genome-wide methylation difference of the longest muscle of the sheep's back of different breeds of sheep,and laid the foundation for the analysis of the differences in sheep skeletal muscle development.In this study,one-year-old sheep(Tan sheep and Hu sheep and Tan-Hu F2) were sanned by the whole genome bisulfite sequencing method(WGBS).The degree of methylation and differentially methylated region in the whole genome DNA of longissimus dorsi(LD) muscle were studied to explore the difference of DNA methylation in level in sheep.The results showed that the methylation (mC) rates of cytosine (C) were 3.55%,3.18% and 3.56% in Tan sheep,Hu sheep and Tan-Hu F2,respectively.A total of 97 731 DMRs and 10 784 related DMGs were detected.In CG,CHH and CHG sequences,the methylation levels of the three groups were not significantly different.419 GO terms and 20 related signal pathways were detected by GO and KEGG analysis,respectively,which were showed to be significantly enriched in cellular process,cell part,binding,long-term depression,and so on.Five candidate genes,ACTA2、ROCK1、CALD1、MYH3 and MYH10 were sreened out in muscle tissues.This study provided genome-wide methylation of pattern of three groups (Tan sheep and Hu sheep and Tan-Hu F2).The results provided reference information for the epigenetic study of Tan sheep and screened candidate genes related to muscle development and meat quality.     

莲雾种质资源遗传多样性的ISSR分析

 对12份莲雾资源和2个莲雾近缘种进行了ISSR分析, 18条引物共扩增出459个位点, 其中多态性位点435个(94.77% ) 。利用UPGMA聚类分析表明: 蒲桃、马六甲蒲桃与莲雾分属于3个不同的组; 12个莲雾品种和品系又分成4个亚组, 这与传统分类学上按照果实成熟期的划分结果基本一致。     

灰飞虱体内沃尔巴克氏体生物学特性及其利用展望

灰飞虱既是传播水稻条纹叶枯病毒的媒介昆虫,也是我国水稻上的主要害虫。Wolbachia是存在于飞虱等多种昆虫体内的一类共生菌,它们能通过多种机制调节寄主的生殖,包括诱导细胞质不亲和、孤雌生殖和遗传上的雄性雌性化等。目前对这类微生物的研究已取得了重要进展。本文综述了Wolbachia在灰飞虱体内的分布、传播方式及其对灰飞虱种群的影响,并初步探讨了利用其生物防治的可能性。     

Resistance mechanisms and associated mutations in acetylcholinesterase genes in Sitobion avenae (Fabricius)

Wheat aphid, Sitobion avenae (fabricius), is one of the most important wheat pests and has been reported to be resistant to commonly used insecticides in China. To determine the resistance mechanism, the resistant and susceptible strains were developed in laboratory and comparably studied. A bioassay revealed that the resistant strain showed high resistance to pirimicarb (RR: 161.8), moderate reistance to omethoate (32.5) and monocrotophos (33.5), and low resistance to deltamethrin (6.3) and thiodicarb (5.5). A biochemistry analysis showed that both strains had similar glutathione-S-transferase (GST) activity, but the resistant strain had 3.8-fold higher esterase activity, and its AChE was insensitive to this treatment. The I₅₀ increased by 25.8-, 10.7-, and 10.4-folds for pirimicarb, omethoate, and monocrotophos, respectively, demonstrating that GST had not been involved in the resistance of S. avenae. The enhanced esterase contributed to low level resistance to all the insecticides tested, whereas higher resistance to pirimicarb, omethoate, and monocrotophos mainly depended on AChE insensitivity. However, the AChE of the resistant strain was still sensitive to thiodicarb (1.7-fold). Thus, thiodicarb could be used as substitute for control of the resistant S. avenae in this case. Furthermore, the two different AChE genes cloned from different resistant and susceptible individuals were also compared. Two mutations, L436(336)S in Sa.Ace1 and W516(435)R in Sa.Ace2, were found consistently associated with the insensitivity of AChE. They were thought to be the possible resistance mutations, but further work is needed to confirm this hypothesis.